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Do Health Promoting Compounds of Flaxseed Attenuate Weight Gain via Regulation of Inflammation?
Author(s) -
Mann Michael,
Wiltgen Ashton,
Silvernagel Kelsie,
Rhee Yeong
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.974.9
Subject(s) - inflammation , tumor necrosis factor alpha , weight gain , obesity , cytokine , medicine , body weight , endocrinology , tumor necrosis factor α , chemistry
Obesity increases pro‐inflammatory cytokine production leading to systemic inflammation. This study determined the effect of health promoting compounds of flaxseed on weight gain and inflammatory cytokine productions. Sixty C57BL/6J male mice were randomly assigned into one of the following diet groups and fed for eight weeks: 1) 45% Kcal fat; 2) 45% Kcal fat, 10% whole flaxseed; 3) 45% Kcal fat, 6% defatted flaxseed; 4) 45% Kcal fat, 4% flaxseed oil; and 5) 16% Kcal fat. Weight and dietary intake were measured weekly. Pro‐inflammatory cytokines, tumor necrosis factor (TNF)‐α and interleukin (IL)‐6, and nucleus factor (NF)‐κB expression in mouse liver samples were determined by real‐time quantitative PCR. The fold change in TNF‐α and IL‐6, and NF‐κB expression was calculated using 2‐ΔΔCT method. The average weight at pre‐treatment among diet groups was significantly different (p=0.013). The weight gain between pre and post diet treatments (p<0.0017) and the average weight at post‐treatment were significantly different (p<0.0001) among diet groups. Although there were no significant effects of diet treatment on selected gene expression, a positive correlation between TNF‐α and NF‐κB expressions (p<0.0001) and a positive trend between TNF‐α expression and weight gain (r=0.23324, p=0.075) were observed. The results indicated a possible mechanism of weight gain regulation by health promoting compounds of flaxseed via control of inflammation, which needs further study. Support or Funding Information NDSU HDE College and HNES Department