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The inhibitory effect of Gomisin N on lipid accumulation during adipocyte differentiation
Author(s) -
Choi SeoYun,
Lee YeonJoo,
Hwang JiHyun,
Choi Jia,
Koh EunJeong,
Song JiHyeon,
Seo YoungJin,
Chei Sungwoo,
Park MinKi,
Lee BooYong
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.974.11
Subject(s) - oil red o , adipogenesis , adipocyte , lipid droplet , 3t3 l1 , endocrinology , medicine , western blot , chemistry , lipid metabolism , lipogenesis , lipid accumulation , obesity , biology , adipose tissue , biochemistry , gene
Obesity is an energy balance disorder. It causes cardiovascular diseases, diabetes mellitus type 2 and certain types of cancer. These relationships rely on the excessive differentiation and proliferation of adipocytes, which leads to increased size and number of fat cells. It caused by over expression of lipogenic enzyme or surplus energy accumulation stored as TG in adipocytes. Inhibition of the adipogenesis and lipid accumulation in 3T3‐L1 adipocytes contributes crucial therapeutic effect on prevention of obesity. In this study, we focused on anti‐obesity effect of Gomisin N (GN) in 3T3‐L1 adipocytes during differentiation. Accumulated lipid droplets were examined by Oil red O staining (ORO). The expression of adipogenic transcriptional gene were subjected by qPCR and Western Blot. The cells were incubated in the presence or absence of GN during differentiation. ORO showed that lipid accumulation was inhibited by GN in dose‐dependent manner. Moreover, the expression of adipogenic and lipogenic factors were suppressed by GN. Our data suggests that GN has an inhibitory effect of lipid accumulation in 3T3‐L1 cells.