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Dietary delta‐tocotrienol modifies serum metabolite profiles in diet‐induced obese mice
Author(s) -
Shen ChwanLi,
Kaur Gurvinder,
Ramalingam Latha,
Tomison Michael D,
Chung Eunhee,
MoustaidMoussa Naima,
Dufour Jannette,
Mo Huanbiao,
Watkins Bruce A.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.972.4
Subject(s) - medicine , endocrinology , insulin resistance , metabolite , glucose homeostasis , diet induced obese , obesity , homeostasis , chemistry , glucose tolerance test , insulin , methionine , amino acid , biochemistry
Obesity with related complications is a world‐wide health problem. Dietary tocotrienols (TT) have been shown to improve obesity‐associated metabolic disorders, such as insulin resistance, in preclinical studies. Here, we aimed to characterize the metabolic status of obese mice by LC/MS‐based metabolomics in combination with relevant biochemical parameters of glucose homeostasis. Twenty male C57BL/6J mice (6‐week‐old) fed either a high‐fat diet (HFD, n=10) or HFD supplemented with 800 mg TT/kg (HFD+TT, n=10) for 14 weeks. TT was a mixture of 90% delta‐TT and 10% gamma‐TT. Compared to the HFD group, TT supplementation improved glucose homeostasis as determined by both glucose tolerance test (at 120 min, 369.3±22.6 and 243.6±38.9 mg/dL for HFD and HFD+TT, respectively) and insulin tolerance test (at 12 weeks relative to time zero: 53.1% and 44.4% for HFD and HFD+TT, respectively). For serum metabolites, the HFD+TT group had higher levels of some essential amino acids, B vitamins, and 12 HEPE in serum compared to the HFD group. In addition, the HFD+TT group had reduced thromboxane B2 and 13 HODE, and increased 12HEPE, lysine, and methionine. These results suggest TT supplementation lowered inflammation and improved macronutrient metabolism in obese mice that may explain reduced obesity. This study demonstrates that the beneficial effect of TT on glucose homeostasis is associated with changes in several metabolite profiles in obese male mice with insulin resistance. Support or Funding Information American River Nutrition, Inc., Hadley, MA.