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Microencapsulation of Lactobacillus salivarious Li01 for enhanced storage viability and targeted microbiome delivery
Author(s) -
Yao Mingfei,
Li Bo,
Xiao Hang,
Li Lanjuan
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.965.8
Subject(s) - probiotic , gelatin , food science , gastric fluid , lactobacillus , digestion (alchemy) , chemistry , bacteria , biology , biochemistry , chromatography , genetics , fermentation
Probiotics, used in food products, dietary supplements and pharmaceutical products have been considered to provide health‐promoting effects in humans. In order to be efficacious, probiotics need to be viable at sufficient abundance in the intestine. However, many functional foods containing probiotics suffer from a large loss of bacterial viability during shelf storage and gastrointestinal transit after ingestion. In this study, probiotic Lactobacillus salivarious Li01 was incorporated into alginate or alginate‐gelatin microbeads. Their morphology was characterized by SEM. Probiotic viability was determined under aerobic conditions, heat treatment and simulated gastrointestinal conditions. The results showed that microbeads were roughly round and almost all the bacterial cells were encapsulated in the microbeads. Encapsulation significantly enhanced the viability of the probiotic during aerobic storage. Moreover, the microbeads maintained their structures during simulated gastric digestion before they dissolved during simulated digestion in the small intestine. Most importantly, the alginate‐gelatin microbeads protected encapsulated bacteria during simulated gastrointestinal digestion and effectively enhanced their survival rate in comparison with alginate microbeads and non‐encapsulated bacteria. In conclusion, the alginate‐gelatin microbeads showed a great potential to facilitate the delivery of probiotics such as lactobacillus salivarious Li01 in various products. Support or Funding Information Chinese High Tech Research and Development (863) Program (2012AA020204, 2013AA020102), the National S&T Major Project (2012ZX10002004‐001)