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Pre‐hispanic Mexican diet reduced dysbiosis and inflammation mediated by LPS in diet induced obesity
Author(s) -
AvilaNava Azalia,
AguilarLópez Miriam,
SanchezTood Monica,
Tovar Armando R,
Torres Nimbe
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.965.23
Subject(s) - gut flora , lactobacillus reuteri , akkermansia muciniphila , lactobacillus , akkermansia , bacteroidetes , feces , prebiotic , dysbiosis , biology , bifidobacterium , food science , prevotella , verrucomicrobia , microbiology and biotechnology , biochemistry , bacteria , 16s ribosomal rna , fermentation , genetics , gene
Obesity is considered a chronic inflammation state accompanied of dysbiosis in the gut microbiota. The purpose of the present work was to study the effect of a combination of foods based on the prehispanic Mexican diet (PMD) (corn, black beans, nopal, tomato, chia and pumpkin seeds on parameters associated with inflammation and gut microbiota. Rats were fed a high fat diet for 6 months, after that, a group of rats were fed the PMD during 3 months, and the rest continued with the high fat/sucrose diet (HFS). At the end of the protocol, feces were collected and DNA was extracted for 16S ribosomal gene sequencing using the MiSeq plataform and a blood sample was collected to determine biochemical parameters. Our results showed that PMD modified the composition of gut microbiota increasing the abundance of Lactobacillus reuteri and Prevotella copri by 30‐fold, Bifidobacterium pseudolongum by 17‐fold and Akkermansia muciniphila by 1.5‐fold with respect to HFS group. On the other hand, PMD decreased the abundance of Staphilococcus epidermis by 70 fold, Bacteroidetes eggerthii by 29 fold, Escherichia coli by 25 fold and Helicobacter apodemus by 6 fold compared to rats fed HFS. Changes in the gut microbiota with the PMD were accompanied with a reduction the inflammatory response by decreasing serum lipopolysaccharide (LPS) levels by 52%. Our results showed that the presence of different compounds such as soluble and insoluble fiber, polyphenols and w‐3 fatty acids with a prebiotic effect, and vegetable protein in the PMD are able to modify gut microbiota decreasing inflammation mediated by LPS caused by obesity. Support or Funding Information Financial support by INCMNSZ.