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Docosahexaenoic Acid Improves the Decrement in Antibody Production Upon Influenza Infection of Obese Mice Through the Production of Pro‐Resolving Lipid Mediators
Author(s) -
Guesdon William,
Crouch Miranda,
Kosaraju Rasagna,
Armstrong Mike,
Reisdorph Nichole,
Shaikh Saame Raza
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.964.24
Subject(s) - docosahexaenoic acid , antibody , polyunsaturated fatty acid , immunology , immune system , immunity , antibody titer , humoral immunity , biology , medicine , titer , fatty acid , biochemistry
Obesity is associated with an increased risk of infection and poor response to vaccination, which may be driven by impaired antibody production. Therefore, therapeutic strategies are needed to enhance the humoral response. We previously demonstrated that n‐3 polyunsaturated fatty acids (PUFA) can enhance antibody production to a T‐independent antigen. Therefore, we tested the possibility that the n‐3 PUFA docosahexaenoic acid (DHA) could improve antibody production upon influenza infection in obese mice. To investigate the effect of DHA on humoral immunity, we fed mice for 15 weeks with a control or a high fat diet with or without DHA supplementation followed by infection with influenza A/Puerto Rico/8/34. Our findings reveal that DHA supplementation improved the decrement in hemagglutination inhibition (HAI) titers of obese mice after influenza infection. Mechanistically, DHA did not target B‐cell PPARγ (peroxisome proliferator‐activated receptors) or the DHA‐sensing G‐protein coupled receptor 120 to boost antibody levels. Instead, DHA increased the levels of downstream D‐series specialized pro‐resolving lipid mediators (SPMs), which are known to increase antibody production through the generation of CD138 + antibody secreting cells. Specifically, we identified 14‐HDHA to be elevated upon DHA intervention, which elevated HAI titers upon influenza infection and increased the frequency of CD138 + cells. Overall, the results suggest that DHA may have potential therapeutic applications for improving humoral immunity. Support or Funding Information Supported by NIH R01AT008375 (SRS).