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Effect of chebulic acid on alveolar epithelial to mesenchymal transition in response to the urban particulate matter
Author(s) -
Lee HeeRa,
Pyo Min Cheol,
Yang SungYong,
Lee KwangWon
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.962.17
Subject(s) - mesenchymal stem cell , dichlorofluorescein , chemistry , epithelial–mesenchymal transition , fibronectin , microbiology and biotechnology , in vitro , particulates , cell , cell culture , biophysics , biochemistry , reactive oxygen species , biology , transition (genetics) , genetics , organic chemistry , gene
Urban particulate matter (UPM) is a heterogenous mixture of various types of atmospheric particles. Exposure to UPM leading to adverse health effects is evaluated by using in vitro cultures of human alveolar epithelial cells. Cell viability showed a time‐ and concentration‐dependent decrease when exposed to UPM. UPM induced time‐dependent reactive oxygen species generation in the alveolar epithelial cell as measurements by 2′,7′‐dichlorofluorescein diacetate. NCI‐H441 cells, the human alveolar epithelial cell line, exposed UPM underwent an epithelial mesenchymal transition as evidenced by the up‐regulated expression of the mesenchymal‐specific proteins α‐smooth muscle actin and fibronectin. In addition, the potential role of chebulic acid in regulating UPM‐induced EMT was observed