Premium
FoxO1 localization changes in Cinnamonium cassia treated 3T3‐L1 pre‐adipocytes
Author(s) -
Bova Kaitlin,
Aulthouse Amy,
Stockert Amy
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.944.5
Subject(s) - foxo1 , transcription factor , phosphorylation , endocrinology , insulin , medicine , chemistry , biology , microbiology and biotechnology , protein kinase b , biochemistry , gene
With increased focus on diabetes treatment and prevention, characterizing the mechanisms of action of various alternative treatments becomes increasingly important. Numerous studies have shown that cinnamon from a variety of species lowers both post‐prandial and fasting blood glucose levels. Our research is focused on examining the effects of aqueous extract of Cinnamonium cassia on a variety of signaling mechanisms normally affected by insulin. One such mechanism utilized by insulin is the Akt pathway which leads ultimately to the phosphorylation of FoxO1, a transcription factor involved heavily in metabolism. Nucleation of the FoxO1 factor is dependent on its phosphorylation state. This study is aimed at determining the effects of cinnamon treatment on the FoxO1 localization. Preliminary studies indicate minimal effects on expression level of FoxO1, indicating that if involved, FoxO1 localization is likely the key to lowering blood glucose levels. We completed a multi‐time point study looking at early and later differentiation stages if the 3T3‐L1 cells both in the presence and absence of cinnamon treatment. Cells were divided into two groups, those which were chemically induced for differentiation and those which received no chemical induction. RNA samples were collected from each time point and treatment groups for synthesis of cDNA followed by quantitative PCR. Although changes in expression level were observed, large changes in protein level were not observed and it is unlikely that expression level of FoxO1 plays a role in the observed clinical outcomes. In order to explore the effects of treatment on localization of FoxO1, protein extractions were conducted isolating both the nuclear and the cytosolic proteins. These samples were analyzed by Western blot to identify what effect cinnamon treatment had on nucleation of the FoxO1. Preliminary analysis of these data suggest that cinnamon facilitates nuclear exclusion. Support or Funding Information Ohio Northern University Academic Affairs Summer Research Support Program