Premium
Anti‐hyperglycemic Effect of Arginyl‐fructosyl‐galactose (AFL) in SD Rat Model Induced Lactase Expression Using Milk Administration
Author(s) -
LEE Jungyun,
Choi HwangYong,
Kim YoungCheul,
Apostolidis Emmanouil,
Kim YoungHo,
Kwon YoungIn
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.944.2
Subject(s) - lactase , postprandial , medicine , endocrinology , lactose , galactose , chemistry , jejunum , biochemistry , insulin , biology
Inhibition of small intestinal lactase involved in the digestion and absorption of lactose can significantly decrease the postprandial increase of blood glucose level after a mixed lactose diet. In the course of screening useful these enzyme inhibitors, we purified and identified arginyl‐fructosyl‐galactose (AFL), a novel Amadori rearrangement compound from milk‐related foods using in‐vitro enzyme inhibition assay method. To evaluate the effect and mode of action of AFL on the postprandial hyperglycemia we investigated the inhibitory activities of AFL against rat intestinal lactase relevant to postprandial hyperglycemia. We also investigated the changes in blood glucose and lactase expression levels in SD rat both in lactase‐induced and non‐induced models after 4 weeks induction of lactase using milk administration. Most lactase‐induced rat showed significantly higher inhibition‐sensitivity on AFL than non‐induced rats. Furthermore lactase‐induced rat showed high lactase expression in middle part of intestine, Jejunum while non‐induced rat showed high in upper intestine, Duodenum. Results suggested that AFL inhibits small intestinal lactase effectively, as a target site of pharmacophore and can improve hyperglycemia followed by high consumption of milk‐related foods.