Premium
Relation of Hypoglycemic Activity and the Antioxidant Capacity of Justicia Spicigera Leaf Extracts in Diabetic Rats
Author(s) -
MartínezMora José A.,
MurilloVillicaña Marina,
SalgadoGarciglia Rafael,
ManzoAvalos Salvador,
CortésRojo Christian,
MontoyaPérez Rocío,
SaavedraMolina Alfredo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.944.19
Subject(s) - diabetes mellitus , ethyl acetate , streptozotocin , creatinine , glibenclamide , antioxidant , blood sugar , ethanol , medicine , traditional medicine , chemistry , endocrinology , pharmacology , biochemistry
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia with disturbances in the metabolism of carbohydrates, lipids, and proteins, where there is an absolute or relative deficiency in the secretion and/or insulin action, or both, reducing the quality and life expectancy of those who suffer, as it promotes the generation of various diseases such as renal disease and neuropathies, among others; hyperglycemia being the cause of complications of diabetes by a mechanism involving oxidative stress. Since ancient times, diabetics have used medicinal plants to maintain normal blood sugar levels. Justicia spicigera (J. spicigera) is a branched and medicinal erect shrub that is used to treat diabetes. The objetive of the project is: To analyze the effect of extracts from the leaves of J. spicigera on levels of glucose, urea, creatinine, cholesterol, triglycerides and activity of mitochondrial antioxidant enzymes in diabetic rats. The plant J. spicigera was used to prepare 3 extracts with solvents of different polarity (hexane, ethyl acetate and ethanol) at 100 mg/kg, and administered for 30 days on a daily basis (1ml) orally to diabetic rats streptozotocin induced. Therefore eight groups of rats were assigned: 1) Normoglycemic control, 2) Diabetic control, 3) Normoglycemic + hexane extract, 4) Diabetic + hexane extract, 5) Normoglycemic + extract ethyl acetate, 6) Diabetic + acetate ethyl, 7) Normoglycemic + ethanol extract, 8) Diabetic + ethanol extract. Our study revealed that the administration of ethyl acetate and ethanol extracts of leaves of J. spicigera have hypoglycemic activity in diabetic rats. The intake of ethanol extract increases levels of urea in normoglycemic rats and increases triglyceride levels in diabetic rats. Meanwhile, administration of the hexane extracts, ethyl acetate and ethanolic did not modify urea levels in diabetic rats, however, they did increase creatinine levels of normoglycemic rats. Hexane and ethyl acetate extracts decrease levels of cholesterol and triglycerides in diabetic rats. It can be concluded that, because each solvent drag different types of compounds expected the different effects obtained in normoglycemic and diabetic rats. Support or Funding Information Acknowledgements: The authors appreciate the partial economic support by CIC‐UMSNH (2.10 to RSG; 2.16 to ASM; 2.37 to SMA).