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Comparative Effects of Avocado Oil and a Thiol Reductant on Impaired Complex I Activity in Mitochondria from Diabetic Rats
Author(s) -
Hernandez Manjury Jatziry,
FloresLedesma Claudia Guadalupe,
SánchezBriones Luis Alberto,
AlonsoVelázquez Celeste,
AguilarToral Roxana,
CamposGarcía Jesús,
SaavedraMolina Alfredo,
CortésRojo Christian
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.944.17
Subject(s) - thiol , mitochondrion , catalase , glutathione , medicine , endocrinology , chemistry , antioxidant , diabetes mellitus , biochemistry , biology , enzyme
Diabetes is a chief factor for the progression of non‐alcoholic liver disease, which in turn, has been closely linked to mitochondrial dysfunction and excessive ROS production. Complex I may have a prominent role in the development of liver disease because its inhibition may drive to impaired hepatic fatty acid oxidation via decreased reoxidation of the NADH generated during beta‐oxidation. Complex I activity decreases by oxidation of specific thiol groups of the protein. Previously, we have demonstrated that avocado oil intake increases the concentration of reduced glutathione (GSH) in liver mitochondria from diabetic rats. As GSH is essential to maintain thiol groups of complex I in a reduced state, in the present study we aimed to compare the effects of avocado oil and beta‐mercaptoethanol, a thiol reductant, on complex I activity of liver mitochondria from type I‐diabetic rats. Respiration in state 3 was impaired in diabetic rats when a complex I substrate was tested. This was attributed to a decrease in complex I activity in diabetic rats. 90‐days avocado oil intake prevented these defects. When mitochondria from diabetic animals were incubated with beta‐mercaptoethanol, it was observed a full recovery of complex I activity. Besides, upregulated activity of catalase was detected in mitochondria from diabetic animals, indicating an increase in the mitochondrial production of H 2 O 2 . All these results suggest that diabetes decreases mitochondrial respiration due to an increment in H 2 O 2 levels, causing the inhibition of complex I due to the oxidation of the free thiol groups of the enzyme, with avocado oil preventing complex I impairment probably by preventing thiol oxidation because enhancement of mitochondrial levels of GSH. Support or Funding Information Acknowledgments: this work was supported by a Coordinación de la Investigación Científica ‐ UMSNH grant (CCR).