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Time Course Metabolic Profiling of Senescing Flight Muscles in The Hawk Moth, Manduca sexta
Author(s) -
Wone Beate,
Kinchen Jason M.,
Kaup Elana R.,
Wone Bernard W. M.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.941.4
Subject(s) - diel vertical migration , biology , metabolite , metabolism , manduca sexta , metabolomics , lipogenesis , lipid metabolism , metabolic pathway , biochemistry , botany , bioinformatics , ecology , larva
Biological aging profoundly impairs skeletal muscle function, performance, and metabolism. Because aging muscle in vertebrates has parallels with aging muscle in insects, we conducted a high‐resolution time course metabolic profiling of middle‐age to advanced age dorsolateral flight muscle in the hawk moth, Manduca sexta , to characterize the metabolic changes associated with aging muscles. These muscle samples were analyzed using non‐biased, global metabolomics profiling technology based on UHPLC/MS/MS 2 and GC/MS platforms. A total of 535 metabolites were characterized across diel time (daytime/nighttime) and from middle‐age to advanced age in senescing muscle. We identified a number of differences, including changes in biochemicals related to energetics, extracellular matrix turnover, and polyamine metabolism. Metabolite over representation analyses indicated a greater than 2‐fold enrichment in biotin, fatty acid, purine, polyamine, branched chain amino acid, TCA cycle, glycolipid, and monoacylglycerol synthesis or function. Increased abundances of glycolytic metabolites suggest shifts toward activated glycolysis with age, whereas decreased abundances in acylcarnitines potentially reflect declining beta‐oxidative use. We have also seen a shift towards decreased polyamine metabolism with age, which might have downstream effects such as an age‐related decline in lipid metabolism because polyamines appear to regulate lipid metabolism through autophagy. Significant metabolite changes were detected between the metabolic profiles of males and females, revealing both similarities and differences across diel time and ages. Notably, 43 metabolites showed diel oscillations in their abundance levels in females and this pattern attenuated in advanced age. Males showed much dampened diel oscillations regardless of age, but overall abundances of these metabolites decreased with age for both sexes. Interestingly, biotin levels were elevated in senescing muscle of males. Collectively, our results obtained uncovered a cascade of biochemical regulation events coordinating senescence in muscle. Support or Funding Information This material is based upon work supported by the University of South Dakota start up funds.