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Resveratrol Shortens the Chronological Life Span of Saccharomyces cerevisiae under Dietary Glucose Restriction
Author(s) -
MadrigalPerez Luis Alberto,
OlivaresMarin Ivanna Karina,
CanizalGarcia Melina,
GonzalezHernandez Juan Carlos,
Nava Gerardo,
RamosGomez Minerva
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.941.1
Subject(s) - resveratrol , oxidative phosphorylation , saccharomyces cerevisiae , mitochondrion , biology , respiration , atp synthase , oxidative stress , life span , biochemistry , yeast , gene , botany , evolutionary biology
The resveratrol (3,4′,5‐trihydroxystilbene, RSV) supplementation in mammalian systems has been related with a decrease of symptoms of diseases related with aging and the increase of longevity. However, notwithstanding the growing number of studies performed with RSV in biomedical area, the molecular mechanism by which RSV acts still remains unknown. Lately, it has been proposed that inhibition of the oxidative phosphorylation activity is the molecular mechanism of RSV action. This mechanism suggests that RSV might induce mitochondrial dysfunction resulting in oxidative damage to cells with a concomitant decrease of cell viability and cellular life span. Additionally, RSV might also have a negative impact on the ATP production via mitochondrial respiration; consequently under energy restriction the RSV effect in cellular viability could be more severe. To prove this hypothesis we measured oxygen consumption, mitochondrial membrane potential and hydrogen peroxide (H 2 O 2 ) release to determine the extent of mitochondrial dysfunction, as well as the chronological life span (CLS) of Saccharomyces cerevisiae , which has been accepted as an important model of oxidative damage and aging. All the experiments were performed in a dietary glucose restriction (0.5% glucose) and with five levels of RSV (0, 10, 30, 50 and 100 μM). As expected, we found that RSV supplementation inhibits the basal respiration in all the concentrations assayed. However, we did not found any change in the mitochondrial membrane potential, except in the high‐level of RSV (100 μM) that showed an increase in this parameter. In the H 2 O 2 release experiment, we found an inverted U‐shape dose‐response behavior, with the highest value in the 10 μM RSV and the lowest in 100 μM RSV. Altogether this data indicate that 100 μM RSV might produce a mitochondrial dysfunction, since inhibits the oxygen consumption and increases the mitochondrial membrane potential. Finally, as anticipated, we found that supplementation of S. cerevisiae cultures with 100 μM RSV decreased CLS. Collectively, this data supports the hypothesis that RSV supplementation decreases CLS in a dietary glucose restriction condition as a result of mitochondrial dysfunction. Support or Funding Information Luis Alberto Madrigal‐Perez (PRODEP scholarship ITESCH‐002).