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The Use of Next Generation DNA Sequence to Determine Fungal Diversity on Potato Tubers Treated with 1,4‐dimethylnaphthalene
Author(s) -
Patel Ravi Narendra,
Diaz Rene Alfonso,
Campbell Michael A
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.940.2
Subject(s) - sprouting , biology , dna , horticulture , solanum tuberosum , isolation (microbiology) , food science , botany , biochemistry , microbiology and biotechnology
Prevention of sprouting in stored potato tubers is a major concern for the agricultural and food industries. The compound 1,4‐dimethylnaphthalene (DMN) has been shown to prevent premature sprouting when fogged onto the surface of potato tubers. Treatment of Saccharomyces cerevisiae cultures with DMN resulted in a temporary suppression of growth. Based on that data we speculate that treatment of stored potato tubers with DMN would result in sprout suppression as well as alteration of fungal communities on the surface of potato tubers. The treatment of potatoes was done with DMN or sterile water (control) for two days. The surface of the potatoes consisted of 4.2 ppm of DMN residue after treatment. Periderm (2 cm) samples were quickly frozen in liquid N 2 and stored at −80°C after the treatment. DNA was isolated from frozen tissues using a Power Soil DNA Isolation Kit (mobio.com). Total DNA was measured and prepared for PCR using primers specific to the ITS regions of fungi (F). PCR products were evaluated using gel electrophoresis and sequenced using Illumina technology (Penn State Nucleic Acid Facility). A total of 3.7 million DNA sequences were analyzed using the Mothur (v1.36) software package to determine fungal diversity by alignment to the silva.eukarya.fasta database. Among all samples there were 1124 operational taxonomical units (OTU). It was determined that there is a greater fungal diversity on the control tissue than the DMN treated tubers. This data suggests the DMN alters the fungal diversity on the surface of potato tubers. Support or Funding Information Penn State Behrend, 1,4‐Group