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Detecting Pal Lipoprotein in Gram‐Negative Sepsis Patients
Author(s) -
Farquharson Kara,
Novick Bethany,
Snyder Emma,
Pichichero Michael,
Hellman Judith,
Michel Lea
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.937.4
Subject(s) - sepsis , urine , escherichia coli , western blot , pathogenesis , monoclonal antibody , antibody , immunology , medicine , microbiology and biotechnology , biology , gene , biochemistry
The clinical condition of sepsis often results from a bacterial infection, such as Escherichia coli (E. coli), which then leads to a systemic hyper‐inflammatory response. During E. coli sepsis, a lipoprotein, Pal, has been shown to be released from the bacterial cell and is thought to contribute to the inflammatory response in human cells. Evidence suggests that Pal is implicated in the pathogenesis of E. coli sepsis, but Pal has not been detected in sepsis patients. The goal of this work was to detect released Pal in the human serum and/or urine of E. coli sepsis patients to further confirm Pal's role in sepsis. We used purified recombinant Pal to determine accessible detection levels in healthy human serum and urine, and we employed SDS‐PAGE and Western Blot techniques to detect Pal in E. coli sepsis patient samples. Our preliminary results suggest that we can detect a protein of similar molecular weight to Pal, using a monoclonal anti‐Pal antibody, in the urine of several E. coli sepsis patients. Support or Funding Information The Camille and Henry Dreyfus Foundation: Special Grant Program in the Chemical Sciences (L. Michel)