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Kv3.1 and Kv3.4 as tumor hypoxia related voltage‐gated potassium channels
Author(s) -
Song Min Seok
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.934.4
Subject(s) - hypoxia (environmental) , potassium channel , subfamily , chemistry , potassium channel blocker , cancer cell , cancer research , biology , medicine , oxygen , cancer , endocrinology , biochemistry , gene , organic chemistry
Hypoxia is one of the representative characteristics of cancer tissue that differs from normal tissue. Our data demonstrate a close relationship between several oxygen‐sensitive voltage‐gated K + (Kv) channel subunits and tumor hypoxia, as well as an importance of these channels in cancer progression. Kv3.1, Kv3.3, and Kv3.4, which belong to the Kv3 subfamily, were examined as tumor hypoxia‐related Kv channels in A549, MDA‐MB‐231, and HT‐29 cells. All three cells lines highly expressed these channels. We found a cell‐density dependent increment of HIF‐1α, a tumor hypoxia marker, in A549 and MDA‐MB‐231 cells, whereas HIF‐1α showed no similar increment in HT‐29 cells, which grow in an aggregate form. Interestingly, expression of Kv3.1 and Kv3.4 showed the same pattern with HIF‐1α in all three cell lines, and we determined that Kv3.1 and Kv3.4 are tumor hypoxia‐related Kv channels. Furthermore, blood depressing substance (BDS) toxin, a Kv3 subfamily specific blocker, inhibited cancer cell migration and invasion. Our results demonstrate that tumor hypoxia related oxygen‐sensitive Kv channels, including Kv3.1 and Kv3.4, appear to be new therapeutic targets for tumor metastasis.