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3,4′,7‐O‐Trimethylquercetin as a Novel Agent to Inhibit in Vitro Ovarian Cancer Cell Migration and Invasion
Author(s) -
Yamauchi Kosei,
Afroze Syeda Humayra,
Dean DeShaundra M,
Mitsunaga Tohru,
Zawieja David C,
Uddin Mohammad Nasir
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.934.14
Subject(s) - ovarian cancer , plasminogen activator , proliferating cell nuclear antigen , cell growth , western blot , gentamicin protection assay , cancer research , cancer cell , cell migration , cell culture , cell , biology , cancer , microbiology and biotechnology , chemistry , medicine , endocrinology , biochemistry , genetics , gene
Objective Quercetin is a representative flavonoid that presents widely in variety of foods and medicinal plants. So far quercetin has been reported to inhibit migration and invasion of cancer via decreasing the matrix metalloproteinase (MMP)‐2. 3,4′,7‐O‐trimethylquercetin (34′7TMQ) was synthesized from rutin as a starting material in order to evaluate the anti‐migration and anti‐invasion activity of ovarian cancer cells. Study Design We evaluated the effect of 34′7TMQ on three ovarian cancer cells SK‐OV‐3, CRL‐1978, and CRL‐11731 function in vitro. Each Cell lines were treated with different concentrations of 34′7TMQ (0.0, 3.125, 6.25 and 12.5 μM). For each cell line cell proliferation, migration and invasion were measured by using a CellTiter Assay (Promega), Cytoselect Assay (Cell Biolabs) and by using a FluoroBlock Assay (BD) respectively. Proliferating Cell Nuclear Antigen (PCNA) was also evaluated in cell lysates of 34′7TMQ‐treated these 3 ovarian cancer cells by western blot analysis. Expressions of urokinase plasminogen activator (uPA) and plasminogen activator inhibitor 1 (PAI‐1) and matrix metalloproteinase‐2 (MMP‐2) were also evaluated in 34′7TMQ‐treated ovarian cancer cells by western blot analysis. Statistical comparisons were performed using analysis of variance with Duncan's post hoc test. Results 34′7TMQ significantly inhibited migration and invasion of three ovarian cancer cells. 34′7TMQ had no effect on proliferation and PCNA expression in ovarian cancer cells. Moreover, 34′7TMQ significantly inhibited the expressions of uPA and MMP‐2 for all ovarian cancer cells. Conclusion These data indicate that 34′7TMQ inhibited migration and invasion of ovarian cancer cell without inhibiting the proliferation. 34′7TMQ may inhibit the metastasis activity of ovarian cancer cell by decreasing uPA and MMP‐2 expression. 34′7TMQ might be further investigated as a novel anti‐ovarian cancer agent. Support or Funding Information The Discovery Foundation (MNU), Grimes Foundation Endowment Award (MNU), Japan Society for Promotion of Science (JSPS) (KY)