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Dissecting the proteome of Drosophila hybrids
Author(s) -
Bamberger Tom Casimir,
Montgomery Miranda,
MartínezBartolomé Salvador,
Yates John R.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.926.2
Subject(s) - proteome , biology , genome , drosophila melanogaster , ploidy , hybrid , proteomics , computational biology , genetics , gene , botany
A cross between two related species generates hybrid animals that are viable, but sterile. Studying hybrids opens up the possibility to interrogate to what degree protein sequence heterogeneity still supports life of cells and organisms. Specifically, hybrid cells with two different haploid genomes need to cope with the extensive number of highly similar proteins that are present at the same time. Here we use quantitative proteomics to dissect the relative contribution of the two species‐specific, haploid genomes to the proteome in D. melanogaster □ D. simulans hybrid flies. The contribution of each species‐specific proteome to the hybrid proteome is quantified with a new software tool ProteinClusterQuant, which accurately represents all peptide to protein relationships. We find that complexity of the hybrid proteome is high during development and later reduced in adult flies. Although genomes of both species are present in an exact ratio of 1:1 in viable hybrid daughter flies, proteins derived from the maternal genome are predominantly found. We conclude that maternally deposited factors guide the expression of the diploid genome towards stability in adult hybrid flies.