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Proteomics and Functional Genomics of the D. grimshawii Sperm and Associated Male Reproductive Tract Tissues
Author(s) -
Karr Tim,
Oses Juan,
Burlingame Alma,
Oliver Brian
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.926.16
Subject(s) - biology , proteome , proteomics , sperm , evolutionary biology , computational biology , functional genomics , genome , drosophila (subgenus) , drosophila melanogaster , genomics , gene , genetics
High throughput shotgun (bottom up) mass spectrometry has become a staple for the study of male reproduction in Drosophila and other diverse taxa. First applied to the Dmel, a growing list of sperm proteomes in mammals and other arthropods is opening up new areas of comparative and functional genomic studies not previously possible. However, very little is known about the proteomes of the associated reproductive tissues, and here we present a proteomic analysis of the D. grimshawii testis (TE), seminal vesicle (SV), accessory gland (AcG) and sperm (SP). These proteomes represent the first comprehensive study of these major components of the Drosophila male reproductive tract. Overall our study identified 4257 total UniProt IDs (1696 TE; 572 SV; 1084 AcG; 687 SP Flybase genes). From this list we obtained 1989 orthologous Dmel IDs for network, genomic and functional analyses. Comparative molecular evolutionary analysis will also be presented demonstating apparent sub‐functionalization of specific GO protein groups. This work establishes a framework for additional studies designed to provide deeper understanding of the evolution of male sexual reproduction traits.