Premium
Interactions of Rat Embryonic Hippocampal Neuronal Stem Cell on Au‐nanowire Arrays and Gold Nano‐Film Surface
Author(s) -
Islam Asiful
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.925.9
Subject(s) - neurite , nanotopography , nanotechnology , materials science , nanowire , nanoporous , hippocampal formation , nano , biophysics , scanning electron microscope , neural stem cell , chemistry , stem cell , biology , in vitro , microbiology and biotechnology , neuroscience , biochemistry , composite material
Hippocampal neuronal stem cell culture on the nano‐substrates has introduced new era in nano‐neuroscience research. Neurons were cultured on the gold nano‐wire and nano‐film surfaces to see the viability and interactions between neurons and nanoscale surfaces. Anodization of aluminum templates created nanoporous alumina tubes arranged parallel to each other and electrodeposition using gold solution (AuHCl 4 ) resulted in arrays of gold nanowires (GNWs) inside the pores. For investigation of Au‐nano‐neuronal interactions we have cultured embryonic day 18 rat hippocampal neurons on the surface of gold nanowires and surfaces. For preliminary understanding of the neuronal growth and connectivity we studied the images of nano‐neuronal interactions by using optical, fluorescence and scanning electron microscopy (SEM). For adhesion of neurons to nano‐arrays and nano‐surfaces we used bioconjugating protein coating Poly‐D‐Lysine (PDL). The rat hippocampus tissues were mechanically dissolved into neuronal cells and cultured on top of the gold nano‐wire (GNW) and flim (GNF) surfaces and preserved at 37° C constant temperatures in a 5% CO 2 incubator. These neurons on the GNW and GNF surfaces started to grow from day 2 and survived up to 25 days or more. The extracellular protein PDL acted as an intermediate substance for neurites growth on the nano films slips. Without a PDL coating layer showed no or very little growth of neurons on the GNF substrates. We demonstrated well‐defined neuronal networks on Au‐nanowire arrays and GNF. The neurons attached with the nanowires surfaces by PDL, which is a positively charged poly‐peptide and plays a vital role in neurites growth on the nanowire arrays. The pre‐and post‐synaptic effects of neuronal interactions on the surfaces of nanowires were measured by Ca+ imaging in keck microscope. This study is the initial result for implementing the gold nano‐substrates to record the electrical signals from the neurons through gold nano‐films or nano‐wires. Support or Funding Information NSF