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Recruitment of Drp1 in Mitochondrial Fission
Author(s) -
Egner John M,
Bakkum Amber,
Gardner Alex,
Hill Blake
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.913.6
Subject(s) - mitochondrial fission , dnm1l , gtpase , microbiology and biotechnology , mitochondrion , mitochondrial fusion , organelle , dynamin , fission , cytoplasm , biology , cell , mitochondrial dna , genetics , physics , endocytosis , gene , nuclear physics , neutron
Intracellular membranes are dynamically shaped by proteins including fission and fusion events important for vesicular transport, organelle trafficking, and cellular homeostasis. Yet how proteins interact with membranes to control these events is largely unknown. We are interested in addressing this question through mechanistic studies of a dynamin superfamily member central to the division, or fission, of mitochondria. Mitochondrial fission is driven by the GTPase mechanoenzyme, Dynamin‐related protein 1 (Drp1). At the mitochondrial surface, Drp1 is thought to assemble into rings around a future site of scission in a GTP‐dependent manner. Curiously, Drp1 is primarily localized to the cytoplasm and how it is recruited to the mitochondrial surface is unknown. Several proteins resident to the mitochondrial surface have been implicated in Drp1 recruitment and subsequent assembly, but the mechanism remains unclear. We are addressing these problems using biochemical, structural, and cell biological approaches. Our results to date will be presented and suggest a new model for Drp1 activity in mitochondrial fission. Support or Funding Information This project was supported by the Clinical & Translational Science Institute (CTSI) through the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant Number UL1TR001436. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Project also supported by NIH grants NIH R01‐GM067180, and NIH R01‐HL128240.