Premium
Characterization of Macromolecular Interactions that Influence Pichia pastoris Vac8p Function in Microautophagy
Author(s) -
Bellmore Jason T.,
Potokar Elijah S.,
Morales Joseph A.,
Patel Shreyas D.,
Fry Michelle R.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.912.10
Subject(s) - pichia pastoris , function (biology) , microbiology and biotechnology , biochemistry , biology , green fluorescent protein , chemistry , gene , recombinant dna
Previous work in our laboratory has demonstrated that Pp Vac8p, a protein required for microautophagy in Pichia pastoris , is an armadillo repeat protein that contains putative fatty acylation sites at the amino terminus. Furthermore, we had demonstrated that Pp Vac8p armadillo repeat domains were essential for protein function in the vacuolar sequestration steps of microautophagy. We have employed immunoblot analysis and fluorescence microscopy to investigate a putative interaction between Pp Vac8‐GFP and actin, a cytoskeletal protein. The results of these studies will help elucidate protein‐protein and protein‐membrane interactions that influence the behavior and function of Pp Vac8p.