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Determining the effect environmental conditions have on the activity of the 3′–5′ exoribonuclease Rrp6
Author(s) -
McAdoo Brooke
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.908.2
Subject(s) - exoribonuclease , exosome complex , rna , biology , microbiology and biotechnology , regulator , biogenesis , non coding rna , long non coding rna , genetics , rnase p , gene
Eukaryotic cells contain quality‐control systems that monitor RNA biogenesis. These systems feature ribonucleases that prevent the accumulation of nonfunctional RNAs as well as regulate normal mRNAs. Rrp6 is a 3′–5′ exoribonuclease that plays a critical role in the 3′‐end formation and degradation pathways in the nucleus. In Sacchromyces cerevisiae Rrp6 physically and functionally interacts with the exosome, a highly conserved RNA‐processing complex. Rrp6 possesses activities that are both dependent and independent on its association with the exosome. It has been well documented that the deletion of RRP6 ( rrp6‐Δ) leads to increased levels of both coding as well as non‐coding RNAs. However few experiments have addressed how changes in nutrient source, temperature, and under hypoxic conditions affect the activity of Rrp6, specifically its ability to regulate coding and non‐coding RNAs. To address this question we evaluated the level of several Rrp6 regulated RNAs in wild‐type and rrp6‐Δ cells grown in media containing either galactose or glucose, grown at 30 degrees and 37 degrees and in the presence or absence of oxygen. We calculated the growth rate of the yeast under these conditions and used quantitative real‐time polymerase chain reaction (qRT‐PCR) and ΔΔ Ct analysis to determine changes in RNA expression. The goal of these experiments is to better understand the activity of Rrp6p, which is critical regulator of RNA levels in all eukaryotes. Support or Funding Information Supported by internal funding by St. John Fisher College.