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Temporal Distribution of EPAC‐1 and EPAC‐2 on Normal Skin and Cutaneous Wound Healing of Chronically Stressed Mice
Author(s) -
RomanaSouza Bruna,
Santos Jeanine Salles,
Schmidt Martina,
MonteAltoCosta Andréa,
Porto Luis Cristóvão
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.904.10
Subject(s) - wound healing , medicine , dorsum , contraction (grammar) , adenylyl cyclase , endocrinology , anatomy , surgery , stimulation
Psychological stress‐induced catecholamines impair cutaneous wound healing through engagement of different pathways. Recently, it was demonstrated that beta‐adrenoreceptor driven generation of cyclic adenosine monophosphate (cAMP) signals via adenylyl cyclase and exchange protein directly activated by cAMP (EPAC) in skin wound healing. However, it has not been shown yet if chronic stress‐induced high catecholamine levels could alter the expression of EPAC‐1 and EPAC‐1 in cutaneous wound healing. Thus, our aim was to observe the temporal distribution of the EPAC‐1 and EPAC‐2 in normal skin and full‐thickness excisional wounds of chronically stressed mice. Adult male mice (n=15) were submitted to a rotational stress which psychological stress model applied in rodents. For this, animals were daily spun at 115 rpm for 15 min every hour during 24 h, until euthanasia. Other animals (n=15) did not submit to stressful environment. Three days after stress protocol, two full‐thickness cutaneous lesions (8‐mm diameter) were created in the animals' dorsum. The wound contraction was macroscopically measured. Lesions were collected 3, 7 and 10 days after wounding. This study was approved by the Ethical Committee for Animal Use of the State University of Rio de Janeiro (n° 027/2014). The macroscopic analysis showed that chronic stress delayed wound contraction when compared to control group 3, 7 and 10 days after wounding. The protein EPAC‐1 expression was greater in the normal skin of stressed mice than in the normal skin of non‐stressed mice. In the wound area, protein EPAC‐1 expression was diminished stress group 7 and 10 days after wounding when compared to control group. The protein EPAC‐2 expression was decreased in the normal skin and wound area of stressed mice after 7 and 10 days when compared to non‐stressed mice. In conclusion, stress alters the protein expression of EPAC‐1 and EPAC‐2 on normal skin and cutaneous wound healing of mice. Support or Funding Information The Coordination for the Improvement of Higher Education Personnel (CAPES) and the National Council of Research (CNPq)