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The Effects of Bone Morphogenetic Protein‐7 and Interleukin‐1 on Biomechanical Properties of Meniscus Explants
Author(s) -
Taylor Victor Wayne,
Hicks Justin,
Ferguson Cristin,
Danelson Kerry
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.902.20
Subject(s) - explant culture , meniscus , chemistry , bone morphogenetic protein , anabolism , andrology , anatomy , biomedical engineering , medicine , in vitro , biochemistry , physics , incidence (geometry) , optics , gene
The meniscus is a wedge, C‐shape fibrocartilaginous tissue that is essential for the normal biomechanical function of the knee. Damage to the meniscus leads to degenerative changes and loss of function mechanical function of the knee. Cytokines like interleukin‐1 (IL‐1) can inhibit repair process. However, anabolic factors like bone morphogenetic protein‐7 (BMP‐7) may help aid in the repair process. The goal of this project is to observe the effects of BMP‐7 and IL‐1 on the biomechanical properties of the meniscus using microindentation test. Method Six‐millimeter porcine meniscus explants were cut using a biopsy punch and cultured in 1% fetal bovine serum media for seven days. Explants were cultured without treatment (control group) or treated with BMP‐7, IL‐1 alpha, and or combined with BMP‐7 and IL‐1 alpha. Microindentation test was used to test the elasticity of the explant tissues. Results The elasticity for the explants treated with BMP‐7 (2.480 MPa) were significant compared to explants treated with either IL‐1 alpha (1.501 MPa; p= .001) or both BMP‐7 and IL‐1 alpha (.866; p ≤ .000). This indicates BMP‐7 and IL‐1 have a significant effect on the meniscus biomechanical properties. BMP‐7 increase the elasticity (but not significant) compared to the control explants (2.072 MPa; p= .065), while IL‐1significantly decrease the elasticity (p= .017). Conclusion This data shows that BMP‐7 has the potential to exert an anabolic effect on cultured meniscus explants reflected by the presence of increased mechanical compression properties. As a result, the meniscus explant tissues that were cultured with BMP‐7 became more elastic after being cultured for seven days. In addition, meniscus cultured with IL‐1 alpha or a combination of BMP‐7 and IL‐1 alpha became less elastic. Future experiments will continue testing the effect of these two treatments.