Premium
Evaluation of Toxicological Effect of Pausinystalia yohimbe Extract on Histology of Kidney of Adult Male Wistar Rat
Author(s) -
Ugochukwu Chioma,
Ihentuge Churchill,
Okechukwu Hope
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.899.6
Subject(s) - histology , kidney , medicine , perfusion , necrosis , yohimbine , endocrinology , pathology , physiology , antagonist , receptor
Pausinystalia yohimbine has been used for the treatment of sexual side effects caused by some antidepressants, as a blood pressure boosting agent, for dry mouth, and for nervous system dysfunction. Twenty adult male wistar rats weighing between 150g to 200g were used in this research to determine the effects of Pausinystalia yohimbe on the histology of kidney. They were divided into five groups; A, B, C, D and E of four rat each. Group A served as control and was fed with feed and water only. Groups B, C, D and E were administered with extract of Pausinystalia yohimbe at a daily dose of 3mg/kgbw, 5mg/kgbw, 7mg/kgbw and 9mg/kgbw respectively for 21 days. At the end of the experiment, the animals were sacrificed by cervical dislocation after anaesthetized with chloroform. The organ of study (kidney) was harvested and sections cut for histological preparation using H and E staining method. Results showed poor perfusion of renal tissue with moderately to severe damage of renal architecture indicating severe fatty change, tubular necrosis, depletion of glomeruli, moderate clumping and distortion of renal architecture and intra renal hemorrhage in all the experimental groups. The severity was more in groups D and E indicating that Pausinystalia yohimbe is capable of causing renal damage in a dose dependent manner