Premium
Colonic mucosa is affected by gastric restriction in an animal model
Author(s) -
Fontes Luiz Gustavo,
Itezerote Ana,
Saleh Samir,
Hojaij Flávio,
Andrade Mauro,
Martinez Carlos Augusto Real,
Akamatsu Flávia Emi,
Jacomo Alfredo Luiz
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.898.9
Subject(s) - h&e stain , stomach , analysis of variance , goblet cell , medicine , atrophy , gastroenterology , animal model , foveolar cell , pathology , gastric mucosa , biology , staining , epithelium
Gastric reduction procedures are currently employed to treat morbid obesity and there are few reports concerning the consequent effects in large intestine. We devised an experimental model of gastric restriction using rats. The animals were submitted to surgical gastrostomy and a cylindrical loofah is inserted in the stomach. Surgical procedure is described in detail elsewhere. We studied 30 adult male Wistar rats divided in three groups: the stomach reduction group (R10), the sham group (S10), which underwent the same procedure except for the loofah insertion and the control group (C10). Animals were fed and kept in separate cages. They were weighed every other day until being sacrificed on day 10. Sections of the colon were obtained and stained with hematoxylin‐eosin and analyzed for the length of the crypts, mucous cells and inflammatory cells. The data were submitted to the Kolmogorov‐Smirnov test to verify the type of distribution. Data with normal distribution are presented as mean ± standard deviation. In this case, to compare groups, variance analysis was performed (ANOVA) followed by Bonferroni multiple comparisons. The tests were performed with 5% significance level. The analysis of goblet cells count on R10 group indicated a statistically significant reduction of goblet cells and the crypts were shallower as compared to the C10. There were no difference regarding inflammatory cells. These findings might be a result of colonic mucosae atrophy secondary to the effects of gastric restriction in this model.