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Evaluation of Effect of Pausinystaila yohimbe extract on Liver of Adult Male Wistar Rat
Author(s) -
Okechukwu Hope,
Ithentuge Churchill,
Ugochukwu Chioma Olejuru,
NDUKWE GODWIN
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.898.4
Subject(s) - histology , yohimbine , liver enzyme , liver tissue , h&e stain , medicine , immunohistochemistry , traditional medicine , andrology , endocrinology , receptor , antagonist
Pausinystalia yohimbe is a psychoactive plant that contains the alkaloid yohimbine. It is widely distributed over the counter as an herbal aphrodisiac. Twenty adult male wistar rats weighing between 150g to 200g were used in this research to determine the effects of Pausinystalia yohimbe on the histology of liver. They were divided into five groups; A, B, C, D and E of four rat each. Group A served as control and was fed with feed and water only. Groups B, C, D and E were administered with extract of Pausinystalia yohimbe at a dose of 3mg/kgbw, 5mg/kgbw, 7mg/kgbw and 9mg/kgbw respectively for 21 days. At the end of the experiment, the animals were sacrificed by cervical dislocation after anaesthetized with chloroform. The organ of study (liver) was harvested and sections cut for histological preparation using H and E staining method. Blood for liver enzyme assay was also collected in sterile bottles. Results showed that the level of liver enzyme biomarkers (AST and ALP) were significantly increased at p<0.05 in groups D and E when compared with the control group A while groups B and C were not significantly increased. There was no significant increase in level of ALT in the experimental groups when compared with the control group. Histologically, the results showed that Pausinystalia yohimbe (burantashi) causes moderate to severe destruction of hepatic tissue with severe infiltrate of inflammatory cells, loss of hepatic tissue, congestion of the blood vessel and portal aggregate of inflammatory cells in groups C, D and E. This shows that Pausinystalia yohimbe is capable of causing hepatic damage