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Histopathologic changes in gastric mucosa after experimental gastric restriction
Author(s) -
Filho Guilherme Mota,
Itezerote Ana Maria,
Saleh Samir,
Hojaij Flávio,
Andrade Mauro,
Martinez Carlos Augusto Real,
Akamatsu Flávia Emi,
Jacomo Alfredo Luiz
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.898.3
Subject(s) - medicine , gastric mucosa , stomach , h&e stain , sham surgery , gastroenterology , pathology , surgery , physiology , staining , alternative medicine
Bariatric procedures play an important role to control metabolic disorders secondary to morbid obesity. Our group developed a technique to simulate gastric restriction in rats in order to establish the physiological effects of these procedures. In this study, we aimed to analyze the histological features of gastric restriction. We used 30 Adult, male Wistar rats (Rattus norvegicus albicans) weighing between 250–350 grams. To produce gastric restriction, The rats were submitted to laparotomy and gastrostomy to insert a cylindrical sleeve Luffa (loofah) (1.5cm in diameter) made of cylindrical luffa, producing experimental phytobezoar. The animals of Sham group (S) were exposed to the same surgical procedure described above, except for the implant of phytobezoar. From the first day after surgery, the animals were divided into three groups of 10 animals each. The group of 10 days until euthanasia was subjected to the stomach reduction surgery (R10) with their controls sham (S10) and normal controls (C10) 10 days. Each animal was weighed every other day until the day of euthanasia. Gastric mucosa in all groups were studied with hematoxylin and eosine and we performed morphometric analyzes of crypts, mucous cells and inflammatory cells. The data were submitted to the Kolmogorov‐Smirnov test and ANOVA followed by Bonferroni or Dunnet T3 multiple comparisons (p<0.05). The experimental group showed deeper crypts and increased counts in either neutrophils and mucous cells as compared to the sham and control groups. The histopathological changes produced by experimental gastric restriction may be related to acute inflammatory reaction caused by the operative procedure and also increased gastric cell secretion of mucus as a compensatory or secondary effect of phytobezoar.