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Classification of Type II Diabetes Mellitus Progression Based on Quantification of Pancreatic Histology
Author(s) -
Papazian Lucine A,
Pan Zenggang,
LaRosa Francisco,
Lee Lisa
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.898.12
Subject(s) - medicine , diabetes mellitus , adipose tissue , pathology , type 2 diabetes , pancreatic islets , kidney , pancreas , disease , islet , endocrinology
Type II Diabetes is a metabolic disorder characterized by insulin insensitivity resulting in a decreased ability to transport glucose into cells systemically. While type II diabetes is well understood both clinically and in the renal system, there is a gap in documentation of the histological changes that occur in pancreatic tissue throughout disease progression. Thus, the objective of this study was to investigate the morphological changes in pancreatic tissues and to establish a set of histological criteria associated with the progression of type II diabetes. The systemic nature of type II diabetes provides opportunity to use the already established renal progression schematic to stage the disease into Diabetic Kidney Disease (DKD) I–IV. In this project, 100 matched pancreatic and kidney tissue slides from autopsy cases, with a pre‐mortem diagnosis of type II diabetes, were obtained from the existing autopsy repository. Kidney tissues were stained with periotic acid schiff to assess alternations in the glomerular basement membrane thus allowing each case to be staged into DKD I–IV. Matched pancreatic tissue slides were digitally scanned using Aperio slide scanner. Each digitized image file was then analyzed using the imagescope software; size and density of pancreatic islets and parenchymal adipose infiltrates were quantified. The quantified metrics and characteristics of the pancreatic tissues were compared across the 4 DKD groups. The results illustrate a general pattern of increased adipose infiltration and decreased islet density that correspond with type II diabetes disease progression. Our findings are consistent with other studies documenting incidental observations of increased adipose infiltration, and beta‐cell deficits in diabetic patients. For the first time, a classification schematic based on pancreatic histology for type II diabetes disease progression, is proposed. Support or Funding Information Capstone Funding MSMHA