Premium
Purple Potato Extract Promotes Intestinal Barrier Function and Differentiation by Activating AMP‐activated Protein Kinase
Author(s) -
Sun Xiaofei,
Du Min,
Navarre Roy,
Zhu MeiJun
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.894.7
Subject(s) - ampk , barrier function , microbiology and biotechnology , protein kinase a , tight junction , klf4 , chemistry , intestinal epithelium , paracellular transport , transcription factor , kinase , biochemistry , biology , epithelium , gene , sox2 , genetics , membrane , permeability (electromagnetism)
Perturbation of the gut epithelial barrier function induces inflammation and other associated health problems. Purple potato contains a high content of polyphenolic compounds that is known for its beneficial effects. The objective of this study was to evaluate the effect of purple potato extract (PPE) on intestinal barrier function and differentiation, and explore its underlying mechanism in Caco‐2 cells. PPE increased transepithelial electrical resistance and decreased FITC‐dextran paracellular flux in Caco‐2 cells, which were associated with strengthened intestinal epithelial differentiation and tight junction assembly. Furthermore, PPE treatment enhanced AMP‐activated Protein Kinase (AMPK) activity, concomitant with increased expression of CDX2, a key transcriptional factor regulating intestinal epithelial differentiation. Knocking down AMPK using the CRISPR/Cas9 system downregulated the positive effects of PPE on intestinal barrier function and differentiation, in parallel with reduced expression of CDX2. In conclusion, PPE improved gut epithelial differentiation and barrier function via activating AMPK. Support or Funding Information Northwest Potato Research Consortium