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CTRP3 Alters Lipid Profile in Response to Ethanol Feeding
Author(s) -
DeGroat Ashley R.,
Clark William Andrew,
Hagood Kendra L.,
Peterson Jonathan M.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.887.11
Subject(s) - triglyceride , steatosis , alcoholic fatty liver , ethanol , chemistry , fatty liver , oleic acid , arachidonic acid , medicine , adipose tissue , endocrinology , liver disease , alcohol , fatty acid , biochemistry , cholesterol , disease , enzyme
The overall goal of this study is to evaluate the role of a novel adipose‐tissue derived cytokine, C1q TNF Related Protein 3 (CTRP3), in preventing Alcoholic Fatty Liver Disease (AFLD) and related complications. We have recently identified CTRP3 as a potential therapeutic treatment for Non‐Alcoholic Fatty Liver Disease (NAFLD). AFLD contributes to nearly half of all liver‐related mortalities, yet the effects of CTRP3 on AFLD have not been explored. To determine the impact of CTRP3 on AFLD we fed wildtype (WT) and CTRP3 transgenic (Tg) mice an ethanol diet ad libitum for 10 days followed by a gavage of a single dose of ethanol (5 g kg −1 ), also known as the chronic plus binge model or “The NIAAA model”. This model mimics the hepatic steatosis and liver injury seen with chronic alcohol consumption. Tg and WT littermates placed on isocaloric control diet (without the addition of ethanol) were used as experimental controls. Results Ethanol feeding resulted in a significant increase in triglyceride accumulation in both WT and Tg mice, with no difference between WT and Tg mice fed the same diet. However, when the lipid content was examined control fed Tg mice had a higher percentage of linoleic acid (18:2 Δ9,12 ) and a decrease in arachidonic acid (20:4 Δ5,8,11,14 ) compared to WT. Whereas on the ethanol diet Tg mice had a ~45% increase in oleic acid (18:1 Δ9 ) compared to ethanol‐fed WT. There was no difference in oleic acid concentration between control‐fed WT and Tg mice. Conclusion Although CTRP3 failed to reduce hepatic triglyceride levels in response to the NIAAA model of AFLD, CTRP3 significantly increased the concentration of the putative anti‐inflammatory and beneficial fatty acid (oleic acid). These findings indicate that although CTRP3 may not prevent alcohol‐induced hepatic steatosis, it may prevent secondary complications associated with AFLD. Support or Funding Information This research was supported in part National Institute On Alcohol Abuse And Alcoholism of the National Institutes of Health under Award Number R03AA023612, East Tennessee State University Research Development Committee (E82262)