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Effects of DHA on Orchidectomy‐Associated Alterations in Vascular Responses
Author(s) -
Villalpando Diva M.,
Campo Lara,
Navarro Rocío,
Largó Carlota,
Muñoz David,
Tabernero María,
Otero Cristina,
García Hugo S.,
Ferrer Mercedes
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.885.10
Subject(s) - endocrinology , medicine , vasodilation , sodium nitroprusside , mesenteric arteries , chemistry , docosahexaenoic acid , antioxidant , polyunsaturated fatty acid , reactive oxygen species , thromboxane , nitric oxide , artery , biochemistry , fatty acid , platelet
The loss of gonadal function induces vascular dysfunction by altering the release and action of NO, prostanoids and reactive oxygen species. Although, the cardioprotective effect of n‐3 polyunsaturated fatty acids has been documented, the specific effect of docosahexaenoic acid (DHA) in vascular function of orchidectomized rats has been scantly investigated. Therefore, the objective of this study was to analyze whether a DHA‐supplemented diet prevents vasodilator and vasoconstrictor alterations induced by impaired gonadal function. For this purpose, mesenteric arteries from control (C) and orchidectomized (CX) 6 months‐old Sprague‐Dawley rats were used. Control rats were fed with a control diet (V‐C), while CX rats were fed with a control diet (V‐CX) or one containing 2% (w/w) DHA (DHA‐CX). Hydrogen peroxide and antioxidant activity were analyzed in serum samples. Mesenteric artery segments were used to analyze: (i) the levels of NO and thromboxane A 2 (TXA 2 ); (ii) the vasodilator response induced by acetylcholine (Ach) in the absence and presence of L‐NAME and indomethacin, the respective inhibitors of NO synthase and cyclooxygenases; (iii) the vasodilator response to the NO donor, sodium nitroprusside (SNP); and (iv) the effect of L‐NAME on the contractile response induced by noradrenaline (NA). The results showed that orchidectomy increased the production of hydrogen peroxide while decreased the antioxidant activity and the DHA‐diet prevented these alterations. The release of NO was decreased in arteries from CX rats and it was restored by the DHA‐diet. The Ach‐induced response was decreased by L‐NAME plus indometacin in arteries from all groups, being the smallest decrease that of arteries from the V‐CX group. In KCl‐precontracted arteries, the SNP‐induced relaxation was greater in arteries from V‐CX group than those of C or CX‐DHA groups. Regarding NA‐induced response, L‐NAME failed to modify that response in V‐CX group, while in arteries from C and CX‐DHA L‐NAME increased the NA‐induced response. These results show that orchidectomy promotes oxidative stress that accounts for a decreased NO and an increased TXA 2 release. The DHA‐supplemented diet in CX rats restored the redox status by decreasing hydrogen peroxide production and by increasing the antioxidant activity; in turn, the production of NO was recovered. Dietary DHA supplementation in CX rats increased the participation of NO in the Ach‐induced vasodilation and in the NA‐induced vasoconstriction. Support or Funding Information Supported by grants (to M. F.) from the Fondo de Investigaciones Sanitarias (PI1100416), Comunidad de Madrid (S2013/ABI‐2783, “INSPIRA1‐CM”) and Fondo Europeo de Desarrollo Regional.