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Impact of treadmill exercise on endothelial function during estrogen deficiency in ovariectomized db/db female mice
Author(s) -
Song Wencong,
Huang Yu
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.883.17
Subject(s) - ovariectomized rat , endocrinology , medicine , estrogen , endothelial dysfunction , diabetes mellitus , endothelium , type 2 diabetes , physical exercise , insulin resistance , sodium nitroprusside , obesity , nitric oxide
Purpose Cardiovascular disease (CVD), obesity and diabetes are major health problem worldwide. The majority of diabetics died of cardiovascular and renal events, largely as the consequence of initial endothelial dysfunction. Postmenopausal women with type 2 diabetes experience higher risks for cardiovascular diseases than age‐matched men and pre‐menopausal women. Current drug therapies have limited effect on the CVD prevention and they also produce side effects and drug resistance. Physical exercise is gaining a growing attention in terms of preventive measure against vasculopathy in obesity and diabetes. This study aims to investigate the possible protective effects of regular physical exercise on vascular endothelial function in ovariectomized diabetic db/db mice. Methods and results We created the ovariectomized (OVX) db/db mice to mimics the postmenopausal state in women with type 2 diabetes, and studied the effects of exercise on restoring of endothelial function and metabolic profile in the OVX db/db mice. Running exercise (10 m/min, 30 min/time, 3 times a week for 4 weeks) resulted in slight improvement of acetylcholine‐induced endothelium‐dependent relaxations without affecting endothelium‐independent relaxations induced by sodium nitroprusside in OVX db/db mouse aortas. Exercise also improved glucose metabolism and insulin sensitivity. qPCR analysis of mouse aortas shows that exercise increased the mRNA expression of SOD2 while it slightly reduced the mRNA of NOX4. Conclusions The preliminary results indicate that regular physical exercise is beneficial to increase anti‐oxidant defense, to improve endothelial function and to enhance insulin sensitivity during estrogen deficiency in OVX diabetic mice. Ongoing experiments will examine the impact of longer period of exercise and different exercise intensity on vascular function, oxidative stress and inflammation, and metabolic function in other metabolic organs such as liver and skeletal muscle. Support or Funding Information Research Grants Council of Hong Kong (CUHK2/CRF/12G, T12‐402/13N), National Natural Science Foundation of China (91339117)