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Longitudinal Strain by Speckle Tracking Imaging Detected Regional Myocardial Dysfunction in a Rat Model of Ischemia‐Reperfusion
Author(s) -
Omoto Ana Carolina Mieko,
Gava Fábio Nelson,
Silva Hadder Batista,
Oliveira Mauro,
Silva Carlos Alberto Aguiar,
Salgado Helio Cesar,
Fazan Rubens
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.846.9
Subject(s) - medicine , ejection fraction , cardiology , basal (medicine) , radial stress , ischemia , artery , heart failure , finite element method , insulin , thermodynamics , physics
The reperfusion therapy after acute myocardial infarction is frequently used for improving coronary blood flow. However, it is known that in long term, reperfused patients may develop heart failure. Echocardiographic follow‐up of reperfused patients has been important to detect cardiac abnormalities. Particularly, strain by speckle tracking has been an echocardiographic approach able to early detect areas of myocardial dysfunction. The aim of this study was to evaluate the endocardial longitudinal strain by speckle tracking in rats submitted to ischemia‐reperfusion (IR). IR consisted of ligation of the left anterior descending coronary artery for 30 minutes, followed by reperfusion. Male Wistar rats were subjected by IR (N=12) or fictitious sham procedure (control, N=9) and were evaluated by echocardiography, under ketamine and xylazine (50mg/kg, 10mg/kg i.p.) anesthesia, after 4 weeks. Echocardiographic videos were obtained in the long axis, with later application of the software analysis of Strain (VevoStrain™ software, Vevo 2100, Visual Sonics) and calculation of the ejection fraction (EF), end‐systolic volume (ESV) and end‐diastolic volume (EDV), on bidimensional mode. The analysis of six myocardial segments revealed difference between groups only on basal anterior region (IR: −12.9 ± 1.8%, control: −17.8 ± 1.3%), and no difference on the other segments: basal posterior (IR: −15.9 ± 3.5%, control: −15.3 ± 2.6%); mid‐posterior (IR: −21.7 ± 3.3%, control: −22.6 ± 2.6%); apical posterior (IR: −23.9 ± 4.2%, control: −25.4 ± 2.8); mid‐anterior (IR: −9.5 ± 1.8%, control: −14.1 ± 2.8%); apical anterior (IR: −16.5 ± 3.3% control: −18.3± 1.6%). There was a reduction in the ejection fraction and an increase in the end‐systolic volume in the IR group (EF: 58.2 ± 4%, ESV: 303.2 ± 46uL) when compared with control group (EF: 74.6 ± 2%, ESV: 133.8 ± 18.7uL). There was no difference in the end‐diastolic volume (IR: 688.7 ± 56.5uL, control: 579 ± 53.7uL). In conclusion, it was found that the evaluation of strain by speckle tracking in a rat model of IR was useful for detecting regional abnormalities in myocardial deformation (as seen in basal anterior region). Future investigations using this technique before four weeks would be important to detect early abnormalities through the speckle tracking in reperfused ischemic myocardium. Support or Funding Information FAPESP and CNPq.