Premium
Adrenal chromaffin cell function in high‐altitude deer mice ( Peromyscus maniculatus )
Author(s) -
Pranckevicius Nicole A,
Scott Angela L,
Nurse Colin A,
Scott Graham R
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.841.7
Subject(s) - endocrinology , catecholamine , medicine , biology , effects of high altitude on humans , chromaffin cell , peromyscus , population , adrenal medulla , acclimatization , hypoxia (environmental) , chemistry , zoology , anatomy , ecology , oxygen , environmental health , organic chemistry
Hypoxia at high altitudes can activate the sympathetic nervous system, which could become maladaptive in high‐altitude natives if it is maintained over chronic periods. The deer mouse ( Peromyscus maniculatus ) has the largest altitudinal range of all North American mammals, with populations living above 4300m elevation. We hypothesized that high‐altitude deer mice might have altered the physiology of chromaffin cells in the adrenal gland to avoid chronic activation of the sympathetic response. We used captive breeding colonies derived from wild populations at high and low altitudes, and compared them in normoxia and after 6–8 weeks of acclimation to hypoxia (12kPa O 2 , simulating the PO 2 at ~4300m). High‐altitude mice show lower rates of catecholamine secretion from adrenal slices (measured via carbon fibre amperometry) in response to stimulation by a nicotinic acetylcholine receptor agonist (nicotine). Here, we used ratiometric Ca 2+ imaging of cultured chromaffin cells to assess whether the variation in catecholamine secretion resulted from variation in the Ca 2+ signal for vesicular catecholamine release. The rise of intracellular Ca 2+ in response to nicotine (1, 5, 25, 125μM) did not vary between populations and was not affected by hypoxia acclimation, suggesting that events downstream of Ca 2+ influx likely contribute to population differences in catecholamine secretion. This appeared to include differences in catecholamine stores, which were lower in highlanders compared to lowlanders in cultured cells from normoxia‐acclimated mice. Our data therefore suggest that chronic sympathetic activation may be avoided in deer mice at high altitudes by a reduction in catecholamine storage and release from the adrenal gland.