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Exercise Increases Brain‐Derived Neurotrophic Factor in Serum Exosomes
Author(s) -
Saengsawang Witchuda,
Kongoun Sasithorn,
Chanda Metha
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.839.5
Subject(s) - microvesicles , neurotrophic factors , brain derived neurotrophic factor , medicine , neurotrophin , endocrinology , neuroscience , central nervous system , biology , microrna , receptor , gene , biochemistry
Brain Derived Neurotrophic Factor (BDNF) is a major neurotrophic factor that elicits several important roles in the nervous system including neuronal development, synaptic plasticity and neuronal survival. It is known that BDNF can be detected in blood circulation and its level is altered during exercise. However, which form of BDNF and how BDNF is released from the brain into blood circulation during exercise are still unknown. Exosomes are small vesicles secreted from cells and carry a number of molecules from one cell to another. Several studies showed that exosomes carry several neuronal proteins and are able to cross blood‐brain barrier. However, it is unknown whether BDNF is present in the exosomes. In the present study, we demonstrated that the truncated form of BDNF was detected in the exosomes isolated form blood obtained from the jugular vein of the horses. In addition, we found that the level of truncated BDNF was significantly higher in the exosomes obtained from the serum of active horses compared to the sedentary horses. These results suggested the possibility that truncated‐BDNF was secreted from the brain into blood circulation through the exosomes. This is the first study demonstrating the presence of truncated BDNF in the serum exsosomes. It also showed the effect of exercise on the level of truncated‐BDNF in serum exosomes. The knowledge can be applied for a better understanding on the roles of BDNF in exercise, as well as in several neurodegenerative diseases, which are related to changes in the level of BDNF. Support or Funding Information This work was supported by a grant from the Thailand Research Fund (TRF) to WS (TRG5880104). We also gratefully acknowledge support from the Faculty of Science, Mahidol University (to WS).