Premium
Ojeoksan suppressed TNF‐α‐induced vascular inflammation in human umbilical vein endothelial cells
Author(s) -
Han Byung Hyuk,
Kim Hye Yoom,
Ahn You Mee,
Jin Xian Jun,
Jeong Da Hye,
Lee Yun Jung,
Lee Ho Sub,
Kang Dae Gill
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.835.11
Subject(s) - umbilical vein , inflammation , cell adhesion molecule , tumor necrosis factor alpha , enos , cell adhesion , human umbilical vein endothelial cell , immunology , vcam 1 , endothelium , medicine , microbiology and biotechnology , chemistry , nitric oxide , nitric oxide synthase , biology , cell , icam 1 , biochemistry , in vitro
Endothelial dysfunction is regarded as an important role in vascular inflammation. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”(), is a well‐known blended traditional herbal formula. OJS is consist of seventeen natural herbal plants. This study was carried out to investigate the effect of OJS on tumor necrosis factor‐alpha (TNF‐α)–induced vascular inflammation in human umbilical vein endothelial cells (HUVECs). Pretreatment of OJS suppressed TNF‐α‐induced expression of cell adhesion molecules, such as intracellular adhesion molecule‐1, vascular cell adhesion molecule‐1, and endothelial selectin in TNF‐α‐stimulated HUVECs. OJS also decreased the adhesion of HL‐60 cells to HUVECs. Moreover, OJS inhibited TNF‐α‐induced matrix metalloproteinase‐2/‐9 (MMP‐2/‐9) activity; significantly decreased TNF‐α‐induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of IκB‐α in the cytoplasm. Pretreatment with OJS also inhibited the translocation of NF‐κB p65 to the nucleus. OJS increased the phosphorylation of endothelial nitric oxide synthase (eNOS) and Akt (protein kinase B) which are related with NO production. In addition, OJS increased the protein expression of Nrf2 and HO‐1 in TNF‐α‐stimulated HUVECs, suggesting its protective effect against vascular inflammation. In conclusion, these results of this study suggested that OJS, the traditional herbal formula, has a protective effect on vascular inflammation. Therefore, OJS might be a potential therapeutic agent for early atherosclerosis. Support or Funding Information This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2008‐0062484).