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Sphingosine‐1‐phosphate reduces adhesion of malignant mammary tumor cells MDA‐MB‐231 to microvessel walls by protecting endothelial surface glycocalyx
Author(s) -
Fu Bingmei,
Zhang Lin,
Zeng Min
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.834.6
Subject(s) - microvessel , glycocalyx , chemistry , albumin , vascular permeability , venule , adhesion , biophysics , pathology , endothelium , biochemistry , biology , angiogenesis , endocrinology , medicine , cancer research , organic chemistry
To test the hypothesis that the plasma phospholipid S1P can reduce tumor cell adhesion to microvessel walls by protecting the endothelial surface glycocalyx (ESG), we quantified the ESG by fluorescence immunostaining of heparan sulfate (HS), the most abundant glycosaminoglycans in the ESG, the microvessel permeability to albumin, and MDA‐MB‐231 tumor cell adhesion in the presence and absence of S1P in post‐capillary venules of rat mesentery. Rats (SD, 250–300g) were anesthetized with pentobarbital sodium given subcutaneously and kept warm on a heating pad. A midline incision (~2 inch) was made in the abdominal wall and the mesentery was gently taken out from the abdominal cavity and arranged on the surface of a glass coverslip for the measurement. A post‐capillary venule of 35–50μm diameter was cannulated by a glass micropipette; after 20 min perfusing with 1% fatty acid free bovine serum albumin Ringer solution with and without 1 μM S1P, the intensity of FITC‐anti‐HS, permeability to albumin and tumor cell adhesion rates were quantified, respectively, in 3 groups of experiments. In the absence of S1P, FITC‐anti‐HS labeled ESG was ~10% of that in the presence of S1P, whereas the permeability to albumin and adherent tumor cells were ~7‐fold and ~3.5‐fold (after 30 min adhesion) those in the presence of S1P, respectively. Our results conform to the hypothesis that protecting ESG by S1P inhibits tumor cell adhesion to the microvessel wall. Support or Funding Information Supported by NIH SC1CA153325.