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The Effect of MCPIP1 Deficiency on Age‐related Lipid Profile Changes
Author(s) -
Yang Chalen,
Deaton Evan,
Chang Yingzi
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.825.14
Subject(s) - triglyceride , medicine , endocrinology , cholesterol , very low density lipoprotein , inflammation , lipid profile , lipoprotein , homeostasis , knockout mouse , chemistry , receptor
Background Chronic inflammation is associated with metabolic disorders, such as impaired lipid homeostasis, which can lead to atherosclerotic plaque formation and ultimately myocardial infarction or stroke. Monocyte chemotactic protein‐induced protein‐1 (MCPIP1) is a negative regulator in inflammatory processes. Previous studies have shown that MCPIP1 gene deficient mice display phenotypic changes, such as splenomegaly, lymphadenopathy, growth retardation, decrease adipose deposit, and increased pro‐inflammatory cytokines. Our current project is to investigate the age‐related lipid profile changes in MCPIP1 deficient mice. Methods Plasma and liver tissues are collected from wild‐type (WT) and MCPIP1 knockout (KO) C57/BL6 mice at ages 2, 4, and 6 weeks. Plasma HDL, LDL‐VLDL, and liver total cholesterol are measured by using HDL and LDL/VLDL quantitation kit from Sigma‐Aldrich. Plasma and liver total triglycerides are quantified by using Cayman Chemical Triglyceride Assay Kit. Results We found that MCPIP1 deficiency significantly: 1) lowered plasma HDL levels in mice at the age of 6 weeks; 2) caused age‐related reduction of HDL‐cholesterol; 3) increased plasma LDL‐VLDL in mice at ages of 2 and 4 weeks; and 4) increased total plasma triglyceride in the mice at the age of 6 weeks. No differences of total triglyceride and cholesterol were observed between WT and KO livers. Conclusion The data collected thus far suggest that MCPIP1 is an important regulator in maintaining plasma lipid homeostasis. MCPIP1 deficiency may contribute to the age‐related changes of lipid profile changes. Support or Funding Information ATSU Masters of Biomedical Sciences Program