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Apelin Increases Airway Reactivity and Inflammation in a Mouse Model of Asthma
Author(s) -
Dholakia Kshiti,
Siddique Armaan,
Kurade Mangesh,
Narke Deven,
Ponnoth Dovenia S.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.820.2
Subject(s) - apelin , medicine , ovalbumin , asthma , inflammation , bronchoalveolar lavage , immunology , proinflammatory cytokine , endogeny , endocrinology , immune system , lung , receptor
There is evidence that inflammatory exacerbations in asthma may be mediated by specific endogenous peptides. Apelin, an endogenous hypotensive and angiogenic peptide, is increased in atopic asthma. However, it is unclear how apelin affects allergic inflammation observed in asthma. We studied the role of exogenously administered apelin in airway hyperreactivity and inflammation in allergic mice. Mice were divided into control (CON) and allergen sensitized‐challenged (SEN) groups. Mice were sensitized (i.p.) on days 1, 6 with 0.2μg ovalbumin (OVA) followed by 5% OVA aerosol challenges on days 11–13. Apelin was given i.p. as a single bolus dose (4mg/kg) on day 14 to SEN mice. Whole body plethysmography (measuring airway responsiveness as enhanced pause, Penh) and bronchoalveolar lavage (BAL) studies were then performed. Airway responsiveness to MCh (48mg/ml) was highest in SEN+APELIN group (149.64±6116 vs. 95.16±30.48% in SEN, p>0.05, n=3). Differential BAL cell analysis showed that treatment with apelin increased eosinophils (68.67±1.86% in SEN vs 80.67 ±1.20% in SEN+APELIN, p<0.05) and neutrophils (8.33±0.88% in SEN vs 13.33±0.88% in SEN+APELIN, p<0.05) in sensitized mice, thus potentiating the allergic inflammation. Although further studies are needed to evaluate detailed cellular mechanisms, the role of apelin and apelinergic system appears to be proinflammatory in this model of asthma. Support or Funding Information LIU start‐up funds (DSP)