Premium
D‐Limonene complexed with β‐cyclodextrin reduces hyperalgesia in a mice model for fibromyalgia
Author(s) -
Barreto Rosana SS,
Lima Pollyana SS,
Araujo Heitor G,
Pereira Erik WM,
Rezende Marilia M,
Guimaraes Adriana G,
Barreto Andre S,
Santos Marcio RV,
Araujo Adriano AS,
QuintansJunior Lucindo J,
Quintans Jullyana SS
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.812.5
Subject(s) - lim domain , chemistry , hyperalgesia , saline , pharmacology , lumbar spinal cord , spinal cord , anesthesia , medicine , nociception , biochemistry , receptor , psychiatry , transcription factor , zinc finger , gene
D‐limonene (LIM) is a monocyclic monoterpene with a lemon‐like odor and is a major constituent in several citrus oils (orange, lemon, mandarin, lime, and grapefruit). The analgesic profile of LIM has been described previously. However, the short half‐life and water insolubility has been a limitation to LIM medicinal uses. So, cyclodextrins, such as β‐cyclodextrin (β‐CD), has been an important tool to minimize these limitations of non‐polar compouns, as LIM. Now, we assessed the anti‐hyperalgesic effect of LIM‐β‐CD complexed or LIM alone in an animal model of chronic musculoskeletal pain. Thus, mice model of musculoskeletal pain was performed as reported by Sluka et al. After we confirmed hyperalgesic behavior, mice were treated with LIM‐β‐CD or LIM (50 mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) or tramadol (TR, 4 mg/kg, i.p.). The mice treated with LIM‐β‐CD showed significant (p<0.001) superior anti‐hyperalgesic effect when compared with LIM alone, these effects can not produced any alteration in the grip strength force. Additionally, we perfused the lumbar spinal cord collected, crioprotected, cut and submitted in an imunofluorescence protocol for Fos protein. Both, LIM‐β‐CD or LIM, significantly (p<0.001) reduced expression of Fos protein on spinal cord horn. Thus, we can suggest that β‐CD improves the anti‐hyperalgesic effect produced by LIM and this effect seems to be due the involvement of descending pain inhibitory mechanisms. Support or Funding Information CNPq, CAPES, FAPITEC‐SE and FINEP/Brazil