Premium
Lasting anti‐hyperalgesic effect of nanoemulsion containing essential oil of H. pectinata in an animal model of fibromyalgia
Author(s) -
Quintans Lucindo Jose,
Quinans Jullyana Siqueira,
Camargo Zaine,
ArrigoniBlank Maria F,
Brito Renan,
Oliveira Marlange,
Santos Marcio,
Araújo Adriano,
Kolker Sandra J,
Sluka Kathleen
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.812.4
Subject(s) - analgesic , essential oil , (+) naloxone , medicine , pharmacology , traditional medicine , chemistry , anesthesia , food science , opioid , receptor
Hyptis species are aromatic plants that produce essential oils which present pharmacologically active substances with antimicrobial, anti‐HIV, analgesic and antiinflammatory activities. We previously demonstrated the analgesic profile of leaf essential oil of Hyptis pectinata , a medicinal plant widely used in Brazilian folk medicine, however, its pharmacological effect is not durable enough for possible applicability in chronic disorders. Thus, we developed and evaluated a nanoemulsion containing leaf essential oil of H. pectinata (NE‐EOH) produces a long‐lasting anti‐hyperalgesic effect on chronic musculoskeletal pain animal model. The NE‐EOH was prepared by addition of 20 wt% Pluronic F127 and showed hydrodynamic diameter and zeta potentials of 220±28 and 10.1±7.2, respectively. Mice were assessed for mechanical hyperalgesia of the paw and muscle (von Frey and tweezer), motor coordination (Rota Rod) and muscle strength (Grip Strength Meter) beginning 1 h after treatment and continuing through 11 days. NE‐EOH, one single treatment (first day only), produced a significant reduction (p<0.05 or p<0.01) of hyperalgesic behavior. This analgesic activity was reversed by both naloxone and methysergide. Moreover, NE‐EOH reduced (p<0.05) substance P levels in neurons from the spinal cord. Thus, NE‐EOH produced a lasting antihyperalgesic effect probably mediated by brainstem inhibitory pathways or reducing excitatory neurotransmitters in the spinal cord. Support or Funding Information CNPq/Brazil [Grants # 441969/2014‐3, 200636/2012‐0 and 470774/2011‐8], FAPITEC/SE/Brazil, CAPES/Brazil and FINEP/Brazil.