Premium
Exploring the Relationship Between Kaiso and C‐Terminal Binding Protein in Breast Cancer in Women of African Ancestry
Author(s) -
CabanUreña Ambar Beatriz,
Jones Alana,
Shin Jeehye,
Park Sam,
GilHernández Sara,
Yan Tingfen,
Kabbout Mohamed,
Liang Genqing,
Byun Jung,
Gardner Kevin
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.809.9
Subject(s) - breast cancer , transcription factor , cancer research , cancer , tissue microarray , immunohistochemistry , epithelial–mesenchymal transition , oncology , medicine , biology , bioinformatics , gene , genetics , metastasis
Breast Cancer is a heterogeneous disease at the molecular and clinicopathological levels and it is well known to be influenced by internal and external factors. Therefore, this study intends to explore the relationship of two important transcription factors that have been observed in several types of cancer: C‐Terminal Binding Protein (CtBP) and Kaiso. CtBP is a transcriptional repressor that has been shown to interact with a wide range of proteins and act as a metabolic sensor through NAD+/NADH levels. On the other hand, Kaiso is a member of the POZ/ZF family that can bind to the methylated CpG dinucleotides or to the non‐methylated sequence, known as the Kaiso binding site (KBS). In previous studies, both transcriptional factors have demonstrated a worse prognosis and survival in cancer patients. Hence, our objectives are determining the relationship and possible molecular links behind these two proteins, by focusing on the transforming growth factor‐β (TGFβ) signaling; an essential pathway in the epithelial to mesenchymal transition (EMT). To accomplish our goal, we have been working with a sample size of 559 breast cancer patients in which we have collected both patient and tumor characteristics and parameters. They include: BMI, adipocyte cross‐sectional area, race, ethnicity, pre or post‐menopausal status, age and mitotic rate. In addition, immunohistochemistry (IHC) was performed using tissue microarrays for: ER, PR, Kaiso, CtBP, pSmad and other molecules associated with TGFβ signaling. Statistical analysis was performed to observe the correlation between the proteins and patient outcome, based on hormone status, race and the activation of the TGFβ signaling pathway. Preliminary results indicate a relationship between Kaiso, CtBP and survival that is modified by race. Further studies are directed at defining the mechanism underlying this relationship.