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GENE EXPRESSION AND COORDINATION OF CELLULAR ZINC TRANSPORTERS AND METALLOTHIONEINS ARE ALTERED IN TYPE 2 DIABETES MELLITUS
Author(s) -
Samman Samir,
Chu Anna,
Foster Meika,
Hancock Dale P,
Petocz Peter
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.802.1
Subject(s) - zinc , transporter , type 2 diabetes mellitus , cohort , biology , population , type 2 diabetes , glucose transporter , metallothionein , endocrinology , diabetes mellitus , medicine , gene , chemistry , biochemistry , insulin , environmental health , organic chemistry
Background/Aims The involvement of zinc in multiple physiological systems requires tight control of cellular zinc concentration. The current study aims to explore the relationships among cellular zinc transporters (ZnT and ZIP) and metallothioneins (MT) in a healthy population and a cohort with Type 2 diabetes mellitus (T2DM). Methods Baseline data from three trials forming two cohorts, healthy (n = 70) and T2DM (n = 42), were used for combined statistical analyses. Cluster analysis and principal component analysis were used to identify groupings within 10 zinc transporter and MT gene expressions of peripheral blood mononuclear cells, stratified by health status. Multiple regression models were used to explore relationships among zinc transporter/MT groupings, dietary zinc and plasma zinc. Results Gene expression of most cellular zinc transporters and MT were lower in the T2DM cohort (P < 0.01). Cluster analysis identified similar groupings of zinc transporters and MT between the cohorts, with the exception of the placement of 2 transporters: ZnT1 and ZIP7 . In the T2DM group, ZnT1 was associated with ZnT6 and ZIP3 , while ZIP7 was associated with ZnT5 , ZnT7 , ZIP1 and ZIP10 . When grouped by the components identified, zinc transporters and MT were significant determinants of plasma zinc (r 2 = 0.48, P = 0.001) in the healthy cohort, but not in T2DM. Conclusions The current study suggests altered cellular zinc homeostasis in T2DM; the dissociation of ZnT1 and ZIP7 from their groupings as identified in the AH cohort, highlights potential defects in zinc transport system associated with T2DM. Support or Funding Information N/A