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Lactate co‐ingested with caffeine stimulates fat oxidation in resting mice
Author(s) -
Yoo ChoongSung,
Kim Jisu,
Kim Heejae,
Hwang Yoonyoung,
Kim Eunseok,
Park Yeram,
Lim Kiwon
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.799.7
Subject(s) - caffeine , chemistry , respiratory exchange ratio , ingestion , respiratory quotient , medicine , lactic acid , carbohydrate , resting energy expenditure , metabolism , endocrinology , energy metabolism , carbohydrate metabolism , biochemistry , biology , heart rate , blood pressure , bacteria , genetics
Lactate is traditionally seen as a fatigue substance. However, there are many beneficial sides of lactate demonstrated in the last decades. Nevertheless, it was not known whether lactate changes on energy metabolism and energy substrates utilization. Accordingly, we investigated the effects of lactate treatment by using an open circuit calorimetry system on resting energy expenditure in resting mice for the duration of 24h. Thirty‐two Sprague Dawley male rats of 7 wk divided into control (Con; DW ingestion), caffeine (Caf; 1mg/100g), lactate (Lac; 400mg/100g), and caffeine and lactate mixed compound (Caf+Lac; 1mg, 400mg/100g) groups. The rats were given the substances to each group by oral administration (Experiment 1). We examined 6 hours on energy metabolism in resting by using respiratory metabolic chambers. There was no significant difference in O 2 uptake and CO 2 production between groups for 6 hr. However, Lac and Caf+Lac ingestions were significantly less than Con in respiratory exchange ratio (RER) over 2 hr after administration. Therefore, in fat oxidation, Lac and Caf+Lac groups were significantly higher than Con group over 2 hours after administration but not change in carbohydrate oxidation. These results suggest that the caffeine and lactate mixed compound and lactate alone groups can improve fat oxidation over 2 hr. Moreover, we performed additional experiment to clarify the mechanism; a time‐based comparison of the effects of caffeine/lactate mixed compound (Experiment 2). We determined the effect of Caf+Lac (1mg, 400mg/100g) based on time. Twenty‐four male SD rats of 7 wk were divided into 4 groups; 0 min, 30 min, 60 min and 120 min after ingestion. We determined MCT1, MCT4, GLUT4, FAT/CD36, CPT1b and PGC‐1α mRNAs in soleus muscle by RT‐PCR. MCT4 mRNA was significantly higher in 30 min and 60 min than in 120 min. GLUT4 mRNA in 120 min was lower than in the other groups. Also, in PGC‐1α in 120 min was significantly higher than in 30 min and 60 min. These proteins could promote fat oxidation by the caffeine and lactate mixed compound.