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Effect of carbohydrate plus branched‐chain amino acids supplementation on hormonal and inflammatory responses during normobaric hypoxic exercise
Author(s) -
Yang YanTing,
Chen ChingHan,
Wu ChiaYing,
Chen ChungYu,
Chou ChunChung,
Bernard Jeffrey R.,
Liao YiHung
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.799.14
Subject(s) - heart rate , medicine , crossover study , endocrinology , carbohydrate , hormone , physical exercise , insulin , chemistry , blood pressure , alternative medicine , pathology , placebo
Purpose Provision of carbohydrate (CHO) and branched‐chain amino acids (BCAA) have been both reported to enhance exercise performance and endurance. However, it is still little known whether the combination of CHO and BCAA on the hormonal and immune cell responses to prolonged exercise under hypoxic environment. Therefore, the purpose of this study was to investigate the effects of CHO plus BCAA supplementation on hormonal changes and immune cell responses during exercise under normobaric hypoxic condition. Methods Using a double‐blind and crossover design, 8 healthy males (22.0 ± 0.5 yr, VO2max = 50.0 ± 1.8 ml·kg–1·min–1, maximal power [Wmax] = 248.0 ± 5.4) were assigned to two trials in randomized orders, including carbohydrate (CHO) and carbohydrate plus BCAA (CHO/BCAA). Participants rested in hypoxic chamber (13.5% O 2 ) for 30 minutes, and then conducted a 50 min moderate‐intensity cycling exercise (5 min warm‐up, 65% Wmax) in the chamber. Heart rate (HR) and oxyhemoglobin saturation (SpO 2 ) were measured every 10 min during exercise. Venous blood samples were collected at baseline, 30 min rest under hypoxia, every 15 min during exercise, and immediately after exercise for the measurements of blood leukocyte subpopulations, blood glucose/lactate, insulin, and cortisol. The two‐way ANOVA with repeated measure was used to compare the differences for all measurements. Results HR and rating of perceived exertion (RPE) began to show a significant increase at 15 min of exercise until the end, and SpO 2 started to decrease at 30 min of exercise until the end. However, there were no differences between CHO and CHO/BCAA trails. The blood glucose concentrations did not differ between trails throughout the exercise period. When exercising under hypoxic condition, the blood lactate showed significant increase at 15 min after exercise until the end of exercise (rest: 0.98 mM; end of Ex: 7.6–7.8 mM; p <0.05), but no difference existed between trails. Cortisol but not insulin had significantly increase immediately after exercise ( p <0.05), whereas no significant differences were observed on these two hormones during exercise between two trials. Among leukocyte subpopulations, only total white blood cells counts showed significantly increase at 15 min after exercise, whereas no significant differences in these hematological parameters (i.e. white blood cell, neutrophils, lymphocyte, and monocyte) during exercise between these two trials. Conclusions This investigation demonstrates that despite increases in HR, RPE, cortisol, and white blood cells when providing CHO during prolonged exercise under normobaric hypoxia, these parameters were not affected by addition of BCAA to CHO. Thus, these results suggest that the supplementation consisting of CHO and BCAA has no effect on further changing inflammatory response when exercising under hypoxia.