Premium
Improvement of Exercise on Diabetes‐Reduced LHR Sensitivity via Increasing Expression of StAR Protein.
Author(s) -
Chou ShihYun,
Tsai ShiowChwen,
Lu ChienChen,
Chen ChiaoNan,
Chen ChihChieh
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.799.13
Subject(s) - endocrinology , diabetes mellitus , medicine , testosterone (patch) , streptozotocin , luteinizing hormone , radioimmunoassay , insulin , hormone
Diabetes mellitus is a highly prevalent chronic disease worldwide. Diabetes patient has suffered from testicular dysfunction including testosterone deficiency. Acute swimming test has been demonstrated the beneficial effect on gonadotropin‐stimulating testosterone via activate cAMP pathway in rats. Purpose The purpose is to assess the effect of streptozotocin (STZ)‐induced diabetes on testosterone production and investigate the mechanisms of endurance training improved on diabetes‐induced testosterone dysregulation. Methods The study used 8 weeks old male Sprague Dawley rats, were divided into four groups, including control, exercise (Ex), diabetes (DM), diabetes combined with exercise (DM+Ex). The rats were intraperitoneally injected with STZ (50 mg/ml, dissolved in citric acid buffer, pH 4.5) to induce diabetes. While the control group were injected with citric buffer. After successfully induced diabetes, endurance running for Ex and Ex+DM rats was carried out on the treadmill for four weeks, and then the animals were sacrificed. The blood were centrifuged, and plasma testosterone (T) and luteinizing hormone (LH) were measured by radioimmunoassays. The Leydig cells were extracted to analyzed expression of LH receptor (LHR) and steroidogenic acute regulatory protein (StAR) by Western blot. All data were presented as mean ± standard error mean. One way analysis of variances (ANOVA) was used to compare the differences for mean of each group. The alpha level was set at 0.05 (p<0.05) for accepting statistical difference for all comparisons. Results The results showed that both groups of STZ‐induced diabetes and exercise training significantly reduced plasma testosterone secretion (p<0.05). Plasma LH were significantly increased in DM group when compared with control group (p<0.01), exercise did not alter plasma LH release, but partially attenuated diabetes‐stimulated LH release (p<0.05). Expression of LHR, extracted from Leydig cells was significantly higher in DM and Ex group than that of control (P <0.05). Compared with control group, expression of StAR protein were also significantly inhibited (P <0.01) in DM group. Ex did not altered the expression of StAR but significantly reversed DM‐inhibited StAR expression (p<0.01). Conclusions The study concluded that diabetes mellitus resulted a lower level of plasma testosterone through decreased the sensitivity of LHR and then decrease the expression of StAR protein and production of testosterone secretion. Exercise is benefit to improve diabetes reduced‐LHR sensitivity via increasing expression of StAR protein. Support or Funding Information MOST I102‐2410‐H‐845‐014‐MY3