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Weight loss due to low‐fat feeding in female mice with obesity induced by high‐fat feeding reduces both body fat and bone status
Author(s) -
Sawin Emily A,
Stroup Bridget M,
Murali Sangita G,
Ney Denise M
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.796.5
Subject(s) - endocrinology , medicine , weight loss , weanling , obesity , chemistry , casein , fat mass , food science
Background Glycomacropeptide (GMP) is a 64 amino acid (AA) glycophosphopeptide released from κ‐casein during cheese making that promotes satiety, reduces body fat, increases bone mass and infers prebiotic and anti‐inflammatory effects. Objective To determine if GMP, when fed as the primary protein source, increases fat oxidation with weight loss and improves body composition and bone status after weight loss compared to casein. Methods Weanling female C57Bl6 mice were fed a high fat casein diet (60% en) for 28 wks and then crossed over to high and low fat diets (HF 60% en; LF 13% en) with casein or GMP for another 10 wks. We assessed body weight, food intake and body composition by DXA. Fatty acid oxidation was measured in liver, muscle, and fat tissues using 14C‐palmitate. Plasma concentrations of amino acids, hormones and cytokines were determined. Bone biomechanical performance was assessed with the 3‐point loading test. Results Mice that were fed the low fat diets after crossover had significantly reduced body mass, body fat, and energy intake compared with the mice that were fed the high fat diets. After weight loss, female mice fed the low fat diets had significantly smaller femoral cross‐sectional area and femoral bone mineral density compared to mice that were crossed over to the high fat diets. Conclusion Crossing over to low fat diets after weight gain on a high fat diet improves body composition by reducing overall body mass and body fat through reduction in energy intake. The reduction in body mass coincides with a reduction in bone status as femoral cross‐sectional area and bone mineral density are reduced. Support or Funding Information Supported by USDA Hatch WIS01790 and a Wisconsin Alumni Research Foundation Accelerator Grant.