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Elevated Muscle Inflammatory Response After Protein‐Dense Food Ingestion in Obese Adults
Author(s) -
Skinner Sarah K,
Beals Joseph,
Van Vliet Stephan,
Niemiro Grace M,
Dilger Anna C,
De Lisio Michael,
Paluska Scott,
Burd Nicholas A
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.794.17
Subject(s) - postprandial , medicine , endocrinology , ingestion , skeletal muscle , insulin resistance , obesity , chemistry , insulin
Skeletal muscle inflammation related to alterations in the toll‐like receptor 4 (TLR4) signaling may lead to insulin resistance and poor muscle remodeling. However, the effect of food ingestion on modulating TLR4/ myeloid differentiation factor 88 (MyD88) signaling with or without excess fat mass has not been examined. Therefore, we examined skeletal muscle TLR4 and MyD88 protein content before and after protein‐dense food ingestion across a range of body mass indexes (BMIs) in healthy, sedentary adults. Eight healthy‐weight (HW: age; 25±1 y, BMI: 23.0±0.4 kg/m 2 , HOMA‐IR: 1.3±0.2), 8 overweight (OW: age; 25±2 y, BMI: 27.3±0.4 kg/m 2 , HOMA‐IR: 1.3±0.1), and 8 obese (OB: age; 29±3 y, BMI; 36.0±1.4 kg/ m 2 , HOMA‐IR: 6.1±0.8) men and women ingested 170 g of lean pork (36 g protein, 3 g fat). Repeated blood samples and muscle biopsies were collected from the vastus lateralis in the basal‐state and over a 5 h postprandial period. Plasma non‐esterified fatty acid (NEFA) concentrations declined during the postprandial period and these values did not differ between groups (P=0.61). Skeletal muscle TLR4 and MyD88 protein content were not different between groups at baseline ( P> 0.05). However, pork ingestion increased muscle TLR4 (1.9‐fold from baseline) and MyD88 protein content (1.6‐fold from baseline) (both P<0.05) in the OB group at 5 h of the postprandial period when compared to the OW and HW groups. These data show that food ingestion modulates the muscle inflammatory effect related to TLR signaling in obese adults, but not overweight and healthy weight adults, and appears to be independent of circulating plasma NEFA concentrations. The elevated muscle inflammatory response to food ingestion may, at least partly, contribute to the impaired postprandial glucose and protein handling commonly observed in obese adults. Support or Funding Information The National Pork Board